Coding

Part:BBa_M36251

Designed by: Evan Masutani, Erica Lieberman   Group: Stanford BIOE44 - S11   (2011-12-09)


AlkS Transcription Factor

AlkS transcription factor is derived from Pseudomonas putida. It typically binds to medium chain length alkanes to change into its activated state. Once in its activated state, AlkS binds to its positively-regulated promoter, PalkB, and permits the sequence downstream of PalkB to be transcribed.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 262
    Illegal EcoRI site found at 475
    Illegal PstI site found at 991
    Illegal PstI site found at 1615
    Illegal PstI site found at 2023
    Illegal PstI site found at 2299
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 262
    Illegal EcoRI site found at 475
    Illegal PstI site found at 991
    Illegal PstI site found at 1615
    Illegal PstI site found at 2023
    Illegal PstI site found at 2299
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 262
    Illegal EcoRI site found at 475
    Illegal BglII site found at 1976
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 262
    Illegal EcoRI site found at 475
    Illegal PstI site found at 991
    Illegal PstI site found at 1615
    Illegal PstI site found at 2023
    Illegal PstI site found at 2299
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 262
    Illegal EcoRI site found at 475
    Illegal PstI site found at 991
    Illegal PstI site found at 1615
    Illegal PstI site found at 2023
    Illegal PstI site found at 2299
    Illegal NgoMIV site found at 729
    Illegal NgoMIV site found at 951
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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